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s: 

Depending on what kind of computations you are doing with MATLAB (and on which toolboxes you are using), Python could be a good alternative to MATLAB.

Python + NumPy + SciPy + Matplotlib are the right combination to start.

For the data, you can, for example, save your data directly in text file (assuming that you are not directly concerned by floating-point precision issues) and read it in Python.

If your data are Excel data, where each value is separated by a ";", you can for example read the file line by line, and use the split() method (with ";" as argument) to get each value.

For MATLAB up to version 7.1, it is possible to directly load .mat files from Python with the scipy.io.matlab.mio module. 

 

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